We offer transient transfection and stable transfection services. Transient transfections are used most commonly to analyze the short term impact of altered gene or protein expression. Plasmid DNA, mRNA, siRNA, and miRNA, are introduced and gene products are expressed in the target cells however the nucleic acids do not integrate into the host cell genome. Therefore, gene product expression is transient and typically results in high expression levels that persist for 24-72 hours when RNA is transfected, or 48-96 hours following DNA transfection. Conversely, in order to analyze the long term impact of altered gene or protein expression investigators typically utilize stable transfection methods to develop stable cell lines that permanently express specific genes through their incorporation in the cellular genome.
We are experienced in scaling-up and/or optimizing transient transfection protocols. We can transiently transfect either your cells or our cells with plasmid DNA, mRNA, siRNA, miRNA, that either you provide or we provide. Transfected cells will be cryopreserved and shipped back to you.
Establishing stable gene expression cell line
Subclone your gene into a suitable mammalian expression vector having one of the usual antibiotic resistance genes, transfect cells, select and screen colonies of resistant cells, grow up and cryopreserve the best clones. Selected and confirmed by Western/dot blot/ELISA and PCR.
- Cell based assay
- Cell based assay development
- Cell line amplification and storage
- Produce cell lysate and cell pellet